DNA Extraction and Purification Process.
Popularly referred to as DNA, Deoxyribonucleic Acid is the element characterizing every living thing. The DNA molecule contains a genetic code that is for every organism, therefore, acting as a blueprint for continuous plans for any substance that the organisms do produce in the course of their lives. Unfortunately as much as it may commonly sound, very few individuals have actually had a chance to see the molecule, this is because the molecule is far too small to easily observe. However, one can simply do extraction on the molecules easily at home or in the school using available household materials like plants and especially peas.
Some of the essential materials you will need for the extraction process include alcohol, meat tenderizer, split peas, dishwashing detergent probably in liquid form, a blender, a beaker or clear glass container, a stirring rod or a spoon. In the first procedure, blend at least a half cup of split peas in a cupful of water. With the soupy mixture you have got from blending, use a homogenous solution to separate the mixture. You may as well consider sparing some of the mixtures just in case. Then, pour some of the solutions you have, an adequate amount of it into a container up to a level of at least two inches.
The third procedure involves adding at least one tablespoonful of the liquid dishwashing detergent on top of the solution. Then consider stirring the mixture slowly for about five minutes. Next consider adding a pinch of the meat tenderizer the stir again for five minutes. Drizzle some alcohol above the solution after stirring. Alcohol should be added until one obtains a layer of three inches on the split peas solution. Peas are particularly considered during the extraction because of their high source of this molecule. During the process, the detergent is simply used to break down the membrane cell hence releasing the cell content and the meat tenderizer is used to dissolve the membrane nuclear for it to release the DNA inside it. The alcohol is used in the process to bring out the DNA strands.
The purification procedure can utilize various processing kits. This simply is attributed by the fact that different samples work with different kind of tissues, forensic samples, plant parts and also different cellular structures. Continuously, there are new inventions being made on machines and cutting edge products through different forms of amplification and facilitations for use in new projects and experiments. Among these ways are the use of magnetic beads and the way happens to be very effective. Another way involves the use of plasmid purification kits which is able to give low endotoxin levels for use in the subject transfection.